MON September 28

Monday, September 28 am - Workshops

Cryo EM Workshop

From the Molecular to the Macro-Scale: Spectroscopic Imaging in the Earth Sciences Across the Photon Spectrum

Metals in Structural Biology I

Science with Femtosecond Pulse Trains

 

  • Cryo EM Workshop*
    • Organizers:  Wah Chiu (Stanford U)
    • Summary:  This session highlights the recent use of cryoEM structure determination of SARS-COVID-2 related macromolecules. Speakers will cover structures of different molecular components of the virus and the cellular interacting partners. Some of talks will describe the impact of the structural results on vaccine and drug developments.
    • Speakers:
      • David Vesseler (U Washington Seattle), Structural studies of the SARS-CoV Spike Glycoprotein
      • David Stuart (Oxford U and Diamond Light Source), The use of cryoEM structural information to advance SARS2-CoV-2 biology and therapy”
      • Erica Saphire (La Jolla Institute for Immunology), Antibodies against Emerging Viral Disease”
      • Rhiju Das (Stanford U), CryoEM of previously unsolvable RNA structures including coronavirus genome elements
      • Stephan Wilkens (SUNY Upstate Medical University, Syracuse), Targeting Vacuolar H+-ATPase for Antiretroviral Therapy
      • Goran Kokic (Max Planck Institute for Biophysical Chemistry, Göttingen), Structure of replicating SARS-COV-2 polymerase”
    • Program Details:  Check back later

*The S2C2 is supported by the National Institutes of Health Common Fund Transformative High Resolution Cryo-Electron Microscopy program.

 

  • Metals in Structural Biology
    • Organizers:  Sarah Bowman (Hauptman Woodward Institute), Jennifer Bridwell-Rabb (U Michigan), Jeney Wierman (SLAC/SSRL)
    • Summary:  Metals play critical roles in biology, conferring unique reactivity, enabling challenging chemistry and redox reactions, and functioning as structural scaffolds. It has been estimated that 30-50% of all proteins bind a metal or metal cofactor. Synchrotron-based techniques enable experiments that measure and monitor metal active site geometry and protein structure, as well as permitting elemental analysis to probe exactly what metals are present in a sample. In the Metals in Structural Biology workshop we will emphasize the use of spectroscopic, X-ray crystallography, XFEL and cryo-EM methods to study metalloprotein structure and function.
    • Panelists, Monday, September 28:
    • Panelists, Tuesday, September 29:
    • Program Details:  Check back later

 

  • From the Molecular to the Macro-Scale: Spectroscopic Imaging in the Earth Sciences Across the Photon Spectrum
    • Organizers: Nicholas Edwards, Sam Webb, John Bargar, Vincent Noel (SLAC/SSRL)
    • Summary:  The aim of this workshop is to address and promote discussion on the scientific and technical needs of the diverse user community that utilizes the x-ray fluorescence imaging beam lines at SSRL. The facilities at SSRL provide analytical capabilities over a wide range of length and photon energy scales, and we plan a schedule of talks to reflect these capabilities. Through this workshop, we hope to raise awareness of this diversity and how current and future beam line developments can continue to enhance and support world class research.
    • Speakers/Program Details:  Check back later

 

  • Science with Femtosecond Pulse Trains
    • Organizer: Dinh Nguyen
    • Summary: Ultrashort intense pulses of x-rays from an x-ray free-electron laser (XFEL) have enabled a host of scientific techniques to probe the ultrafast dynamics of chemical reactions and biological phenomena on the femtosecond timescale. These ultrafast events have been elucidated using pump-probe techniques in which one, or at most two, femtosecond x-ray pulse is used to follow the electronic or nuclear motions created by another ultrafast driver such as a femtosecond optical pulse from a chirped pulse amplified (CPA) laser.  In order to obtain a time-lapse sequence of a dynamical event, the time delay between the CPA driver and the x-ray pulses has to be adjusted in multiple shots in order to map out theentire event.  In the next few years, XFELs will be capable of generating a train of femtosecond x-ray pulses, and a few XFELs, specifically those driven by superconducting linac, will have the ability to generate x-ray pulses at high repetition rates, up to 1 million pulses per second.  These new capabilities are expected to significantly enhance the ultrafast science by allowing the femtosecond x-ray pulses to follow single-shot or non-repetitive events over a large dynamic timescale. The purpose of this workshop is to identify the unique science experiments that will benefit from the advent of a train of femtosecond x-ray pulses with pulse spacing adjustable from tens of femtoseconds to microseconds..
    • Speakers/Program Details
    • Time
      PST
      Title of Talk Speaker (First, Last Name) Institution
      8:00 AM   Agostino Marinelli SLAC
      8:30 AM   Felix Lehmkuehler DESY
      9:00 AM Detector Heinz Graafsma DESY
      9:30 AM Probing material heterogeneities during dynamic compression Richard Sandberg Brigham Young University
      10:00 AM Landscape of new lightsource diagnostics for frontier HED experiments Arianna Gleason Stanford University
      10:30 AM X-ray laser oscillator pumped by fs x-ray pulses from an X-ray FEL Uwe Bergman/Alex Halavanau SLAC
      11:00 AM Generation of multiple fs x-ray pulses in a pulse train Dinh Nguyen SLAC
      11:30 AM Wrap-up Discussion Moderated by Dinh Nguyen SLAC
      12:00 PM